| Round of 32 |
| Logan Radik Maryville University | PIN | John Kramer Lindenwood University |
| Gabriel Rangel Lincoln College | PIN | Alexander Cantu Labette Community College |
| Theo Gerve Ellsworth Community College | PIN | Elisha Eriade Benedictine College |
| Demetrius Thomas Williams Baptist College | DEC 6-4 | Shawn Beeson Northeastern Oklahoma A&M College |
| Christian Robertson Northeastern Oklahoma A&M College | PIN | Dhruv Mitroo Washington University |
| Matt Kent Lincoln College | DEC 3-2 | Gary Whitaker Ellsworth Community College |
| Ben Elledge Northeastern Oklahoma A&M College | DEC 13-7 | Anthony Ottinger Labette Community College |
| Jerhett Lee Ouachita Baptist University | PIN | Onosai Pule Wentworth Military Academy |
| Tommy Helton SIUE | PIN | Mickey Pelfrey Maryville University |
| Dante Jiovenetta MIZZ | DEC 6-5 | Jalen Gowdy Ellsworth Community College |
| Vincent Spalitto OKLA | DEC 8-1 | Dominick Johnson LIB |
| Jacob Bohlken MIZZ | PIN | Derek Lewis Marian University (Ind.) |
| Christian Boyles McKendree University | PIN | Jacob Tanner Wentworth Military Academy |
| Tyler Hearns Lincoln College | PIN | Payton Napoli Missouri Valley College |
| Russell Coats Northeastern Oklahoma A&M College | PIN | Derrick Lenz Ellsworth Community College |
| Ethan Anderson OKST | PIN | Cody Curnutt Drury University |
