| Round of 64 |
| Lance Burkhart Iowa Western Community College | PIN | Tyler Willis Midland University |
| Kevin Cooper Ellsworth Community College | PIN | Travis Kephart Midland University |
| Sam Phillips Iowa State Wrestling Club | DEC 11-10 | Clayton Hintz Concordia (Neb.) |
| Devin Tortorice Unattached | PIN | Thaddeus Rinehart Northwest Missouri State Wrestling Club |
| Renaldo Rodriguez-Spencer ISU | DEC 12-6 | Trevor Fiorucci Iowa Central Community College |
| Jason Alfau Iowa Central Community College | DEC 10-3 | Alex Hernandez ISU |
| Tyrell Galloway NEB | DEC 9-6 | Ruben Burrows MWC Wrestling Academy |
| Ryan Vandall Central College | MAJ DEC 12-0 | Steve Bleise NIU |
| Teddie Harvey Iowa Central Community College | TF 16-1 | Blayne Briceno ISU |
| Seth Stender Midland University | TF 17-1 | Eddie Harvey Iowa Central Community College |
