| Round of 32 |
| Mike Evans IOWA | PIN | Cody Bleaken Cumberland University |
| Dimitri Willis Maryville University | MAJ DEC 12-3 | Daniel Urban Lindenwood University |
| Clayton McFarlane Affliction Wrestling Club | MAJ DEC 13-3 | Conner Lefever Indiana Institute of Technology |
| Christian Loges Maryville University | PIN | Joshua Wells Truman State University |
| Nicolas Haferkamp McKendree University | DEC 10-5 | Kevin Corbelt Affliction Wrestling Club |
| Tyler Prazma MIZZ | PIN | Ethan Henry Cumberland University |
| Bryce Alexander Hannibal-LaGrange University | PIN | Moroni Deanda Wentworth Military Academy |
| Damon Jackson Missouri Valley College | MAJ DEC 8-0 | Cody Willis McKendree University |
| Matt Beasley McKendree University | DEC 7-3 | Andre Foster Lincoln College |
| Daniel Breit Lindenwood University | MAJ DEC 19-8 | Nick Sasfy Cumberland University |
| Nick Moore IOWA | PIN | Andrew Elmore Cumberland University |
