| Round of 32 |
| Walker Slaughter Lindenwood University | PIN | Patrick Cervantes Missouri Valley College |
| Joey Trizzino IOWA | DEC 8-1 | Grayson Smith Indiana Institute of Technology |
| Caleb Gibbens Missouri Valley College | DEC 8-6 | Darnell Thomas Lincoln College |
| Justin DeAngelis OKLA | MAJ DEC 10-1 | Stanton Ketcherside Lindenwood-Belleville |
| Josh Cyr MIZZ | PIN | Ryan Badey UCM |
| Joseph Moorhouse Maryville University | DEC 6-2 | Domonique Hardy Rend Lake College |
| Nico Martinez Missouri Valley College | DEC 10-9 | Kameron James Lincoln College |
| Joe Ducharme IOWA | MAJ DEC 12-2 | Darrin Dunn Lincoln College |
| Brandon Isbell Cumberland University | PIN | Conner Linehan Lindenwood-Belleville |
| Michael Kelly IOWA | PIN | Ryan Packard Missouri Valley College |
| Gian Traverso Missouri Valley College | DEC 11-9 | Trevor Hampton Lindenwood University |
| Derek St. John IOWA | TF 17-2 | David Wilson Lindenwood University |
| Austin Lindsay MIZZ | DEC 7-2 | Zeke Hunt Cumberland University |
| Patrick Rhoads IOWA | DEC 5-1 | Almondo Conner Jr. Maryville University |
| Jake Gingerich ILL | DEC 10-5 | Alex Young Lincoln College |
| Gary Lindsay MIZZ | TF 17-2 | Christopher Murray Missouri Valley College |
